Enzyme Substrates
Proprietary, easy-to-use enzyme substrates and kits
Vector's unique Horseradish peroxidase (HRP) and alkaline phosphatase (AP) enzyme substrates offer:
- Novel color choices — stain antigens in pigmented tissues and melanomas, or localize multiple antigens in a single tissue section
- Visibility under brightfield, darkfield, or electron microscopy
- Unique spectral profiles — useful for antigen colocalization using spectral imaging systems
Our enzyme substrate kits supply the reagents in convenient dropper bottles. This allows you to handle chromogens safely and easily, and eliminates wait times for mixing and dissolving powders or tablets.
Enzyme Substrates / Microscopy Table
This table is designed as a reference to help you determine the optimal mounting medium for your application.
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Type of Microscopy
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| Substrate |
Catalog Number |
Brightfield |
Darkfield |
Electron |
Fluorescence |
Spectral
Imaging |
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Horseradish Peroxidase (HRP)
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DAB (brown)
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SK-4100 |
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DAB-Ni (gray-black)
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SK-4100 |
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ImmPACT DAB (brown)
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SK-4105 |
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ImmPACT DAB EqV (brown)
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SK-4103 |
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Vector VIP (purple)
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SK-4600 |
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ImmPACT VIP (purple)
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SK-4605 |
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SG (blue-gray)
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SK-4700 |
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ImmPACT SG (blue-gray)
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SK-4705 |
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Vector NovaRED (red)
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SK-4800 |
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ImmPACT NovaRED (red)
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SK-4805 |
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AEC (red)
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SK-4200 |
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ImmPACT AEC (red)
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SK-4205 |
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ImmPACT AMEC Red (red)
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SK-4285 |
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TMB (blue)
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SK-4400 |
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Alkaline Phosphatase (AP)
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Vector Red (magenta)
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SK-5100 |
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ImmPACT Vector Red (magenta)
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SK-5105 |
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Vector Black (black)
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SK-5200 |
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Vector Blue (blue)
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SK-5300 |
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BCIP / NBT (indigo)
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SK-5400 |
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Additional Enzyme Substrate Characteristics
This table is designed as a reference to help you determine the optimal mounting medium for your application.
| Substrate |
Catalog Number |
Mounting
Medium |
Contrast in
Pigmented
Tissue |
Multiple
Labelling |
Heat
Resistant |
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Horseradish Peroxidase (HRP)
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DAB (brown)
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SK-4100 |
Non-aqueous
or Aqueous
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•
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DAB-Ni (gray-black)
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SK-4100 |
Non-aqueous
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ImmPACT DAB (brown)
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SK-4105 |
Non-aqueous
or Aqueous
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ImmPACT DAB EqV (brown)
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SK-4103 |
Non-aqueous
or Aqueous
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•
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Vector VIP (purple)
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SK-4600 |
Non-aqueous
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ImmPACT VIP (purple)
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SK-4605 |
Non-aqueous
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SG (blue-gray)
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SK-4700 |
Non-aqueous
or Aqueous
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ImmPACT SG (blue-gray)
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SK-4705 |
Non-aqueous
or Aqueous
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Vector NovaRED (red)
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SK-4800 |
Non-aqueous
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ImmPACT NovaRED (red)
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SK-4805 |
Non-aqueous
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AEC (red)
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SK-4200 |
Aqueous
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ImmPACT AEC (red)
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SK-4205 |
Aqueous
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ImmPACT AMEC Red (red)
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SK-4285 |
Aqueous
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TMB (blue)
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SK-4400 |
Non-Aqueous
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Alkaline Phosphatase (AP)
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Vector Red (magenta)
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SK-5100 |
Non-Aqueous
or Aqueous
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ImmPACT Vector Red (magenta)
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SK-5105 |
Non-Aqueous
or Aqueous
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Vector Black (black)
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SK-5200 |
Non-Aqueous
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Vector Blue (blue)
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SK-5300 |
Non-Aqueous
or Aqueous
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BCIP / NBT (indigo)
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SK-5400 |
Non-Aqueous
or Aqueous
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Browse all Enzyme Substrates
