Description
This kit contains all of the reagents necessary to prepare either a DAB or a DAB/nickel substrate working solution. Vector DAB Substrate (3,3-diaminobenzidine) produces a brown reaction product in the presence of peroxidase (HRP) enzyme. This kit also includes nickel chloride, which, when added to the substrate working solution, results in a gray-black reaction product. Vector DAB Substrate is suitable for darkfield and electron microscopy (EM).
Features of this kit:
- Ideal for IHC/ICC/ISH and blots
- Heat Stable
- Permanent mounting
- Ideal for single and multiple labeling
- No wait times for mixing and dissolving powders or tablets
- Sufficient reagents to produce 300 ml of working solution
Specifications
More Information
Unit Size |
1 kit |
Applications |
Immunohistochemistry / Immunocytochemistry, In situ hybridization, Blotting Applications, Elispot |
Detection Enzyme(s) |
Peroxidase |
Substrate Reaction Product |
Precipitating Reaction Product |
Chromogen Color |
Brown, Gray-Black |
Microscopy |
Brightfield, Darkfield, Electron, Spectral Imaging |
Additional Substrate Properties |
Heat Resistant, Multiple Labeling |
Mounting |
Aqueous (Hardening), Non-Aqueous (Permanent) |
Safety Title |
WARNING: Cancer - www.P65Warnings.ca.gov |
Kit Components
What's in the box:
- 6 ml DAB Reagent 1
- 6 ml DAB Reagent 2
- 6 ml DAB Reagent 3
- 6 ml DAB Reagent 4 (Nickel Optional)

