Counterstains and Special Stains

Histology stains for improved target visualization and localization

Histology stains and counterstains introduce color to specific cellular structures to provide contrast to colored enzyme substrates. This makes targets easier to see, which makes it easier to interpret tissue morphology and cell structure.

Counterstains

Counterstains stain cell nuclei and are packaged as convenient, ready-to-use solutions for use on slides or in staining dishes. Available to you (see selection guide below):

• Vector^® Hematoxylin — based on Gill’s formulation, can be adjusted for optimal intensity; suitable for use with non-aqueous and aqueous mounting media and is mercury- and alcohol-free
• Vector^® Hematoxylin QS — based on a modification of Mayer’s hematoxylin and was developed specifically for immunocytochemistry (ICC). It stains within 45 sec, is suitable for use with non-aqueous and aqueous mounting media, and is mercury-free
• Vector^® Methyl Green — offers a simple, two-step procedure and is an excellent alternative in multiple antigen labeling when hematoxylin obscure substrate color. It is suitable for use with non-aqueous mounting media
• Vector^® Nuclear Fast Red — offers a fast, one-step protocol and is an excellent alternative in multiple antigen labeling when hematoxylin obscure substrate color
• Hematoxylin and Eosin Stain Kit — includes a modified Eosin for cytoplasm staining and Hematoxylin for crisp, intense blue nuclei

Special Stains

Use the Alcian Blue (pH 2.5) Stain Kit to visualize sulfated and carboxylated acid mucopolysaccharides and sulfated and carboxylated sialomucins (glycoproteins).

• Acidic sulfated Mucosubstances (blue)
• Hyaluronic acid: Blue
• Sialomucins (blue)
• Nuclei (red)

Counterstain / Substrate Compatibility

This table is designed as a reference to determine the optimal counterstain / substrate combination for your application. Consideration should be given to tissue type, antigen unmasking protocol, and other detection parameters to achieve the desired staining intensity.

• https://vectorlabs.com/browse/counterstains-and-special-stains